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Inter‐relationship Between the In vivo Metabolism of Apolipoprotein B 100 ‐Containing Lipoproteins and LDL Particle Size and LDL Particle Number
Author(s) -
Leclerc Myriam,
Ooi Esther,
Couture Patrick,
Richard Caroline,
Desroches Sophie,
Marin Johanne,
Tremblay André,
Lamarche Benoît
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.248.3
Subject(s) - apolipoprotein b , medicine , endocrinology , very low density lipoprotein , chemistry , triglyceride , lipoprotein , in vivo , catabolism , adiponectin , albumin , cholesterol , metabolism , biology , obesity , insulin resistance , microbiology and biotechnology
Studies have shown that small dense LDL particles confer an increased risk of coronary heart disease (CHD) compared with large LDL. LDL particle number is also an important risk factor for CHD. The objective was to investigate the inter‐relationship between the in vivo kinetics of apolipoprotein (apo) B100‐containing lipoproteins and LDL particle size (LDLsi) and LDL particle number (LDL‐apoB). This analysis is based on data from 154 male and female subjects among whom in vivo lipoprotein kinetics were investigated using a bolus/infusion of D 3 ‐leucine. LDLsi was assessed by non‐denaturing polyacrylamide gradient gel electrophoresis. Participants' mean age (±SD) was 44.7±12.6 yrs. Mean body mass index (BMI) and triglyceride levels were 28.6±4.7 kg/m 2 and 1.97±1.4 mmol/l respectively. LDLsi correlated positively with plasma adiponectin levels (age and BMI‐adjusted Spearman r=0.41, P<0.001) and the fractional catabolic rate (FCR) of VLDL‐apoB (r=0.43, P<0.001) and negatively with plasma TG (r=‐0.42 P<0.001) and the pool size of VLDL‐apoB (r=‐0.38, P<0.001). Plasma LDL‐apoB levels correlated positively with the production rate of VLDL‐apoB (r=0.27, P=0.006) and negatively LDL‐apoB FCR (r=‐0.59, P<0.001). LDL‐apoB showed no correlation with plasma TG (r=0.07, P=0.47), LDLsi (r=0.18, P=0.08) and adiponectin (r=‐0.02, P=0.82). These data suggest that LDL size and LDL particle number are determined by distinct metabolic pathways. Funding provided by the Canadian Institutes of Health Research and the Chair of Nutrition, Université Laval