Premium
Using Lipidomics to Probe Lipid Metabolism
Author(s) -
Welti Ruth,
Shiva Sunitha,
Vu Hieu,
Roth Mary,
Tamura Pamela,
Samarakoon Thilani,
Colter Madeline,
Sarowar Sujon,
Li Maoyin,
Gadbury Gary,
Wang Xuemin,
Shah Jyoti
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.220.3
Subject(s) - galactolipids , lipidomics , chemistry , biochemistry , lipid metabolism , glyceride , sphingolipid , electrospray ionization , phosphatidic acid , sphingosine , mass spectrometry , chromatography , arabidopsis thaliana , phosphatidylglycerol , phospholipid , phosphatidylcholine , fatty acid , membrane , gene , chloroplast , receptor , mutant
Direct‐infusion electrospray ionization triple quadrupole mass spectrometry method with multiple reaction monitoring (MRM) has been employed to measure Arabidopsis thaliana galactolipids and phospholipids (including monoacyl molecular species, molecular species with oxidized acyl chains, phosphatidic acids (PAs), tri‐and tetra‐galactosyldiacylglycerols (TrGDGs and TeGDGs), head‐group‐acylated galactolipids, and head‐group‐acylated phosphatidylglycerol (acPG)), sulfoquinovosyldiacylglycerols (SQDGs), sphingolipids, di‐ and tri‐acylglycerols (DAGs and TAGs), and sterol derivatives following wounding, cold, and freezing stresses. Changes observed are consistent with activation of lipid oxidizing, hydrolyzing, glycosylating, and acylating activities. Analysis of the lipid changes, their inter‐relationships, and their genetic underpinnings will be discussed.