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Urolithin C, a Gut Microbiota Metabolite Derived from Ellagic Acid, Attenuates Triglyceride Accumulation in Human Adipocytes and Hepatoma Huh7 Cells
Author(s) -
Kang Inhae,
TomásBarberán Francisco,
Carlos Espín Juan,
Chung Soonkyu
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.130.1
Subject(s) - adipogenesis , adipocyte , ellagic acid , metabolite , chemistry , lipid metabolism , biochemistry , gut flora , adipose tissue , polyphenol , antioxidant
Urolithins (Uros) are gut microbiota‐derived metabolites from ellagitannins and ellagic acid (EA), naturally occurring polyphenols abundantly found in pomegranate, tea, some berries, and nuts. EA exerts various health benefits including TG‐lowering effects. Emerging evidence suggests that different Uros are produced in response to host's metabolic health and that each Uros may potentiate or nullify the health benefits of EA. Hitherto, little is known about the impact of individual Uros on lipid metabolism. The aim of this study is to assess potency of individual Uros in regulating lipid metabolism using human adipocyte and hepatoma cell lines. To determine the impact of Uros in adipocyte differentiation, Uro‐A, ‐B, ‐C, ‐D, and isoUro‐A (30mM) were added to differentiating human adipogenic stem cells ( h ASCs). Despite structural similarities, only Uro‐C was effective in reducing adipogenesis evidenced by TG accumulation, adipogenic gene and protein expressions. Consistently, Uro‐C potently inhibited de novo FA synthesis ([ 3 H]‐acetate into [ 3 H]‐FA) and TG esterification ([ 14 C]‐OA into [ 14 C]‐TG) in fully differentiated human adipocytes. Furthermore, Uro‐C, but not the other Uros, significantly attenuated TG accumulation and lipogenic gene expression in human hepatoma huh7 cells, which is comparable to EA treatment. Taken together, our results demonstrated that Uro‐C is the most biologically active gut metabolite of EA exerting potent lipid‐lowering effects both in adipocyte and hepatocytes.