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Sex‐specific Effects of Prenatal and Post‐weaning High Fat Diet on Adult Mouse Offspring Skeletal Muscle Contraction
Author(s) -
Jones Lisa,
Markiewicz Filip,
James Rob,
Cagampang Felino,
Rustighi Emiliano,
Poore Kirsten,
Newland Philip,
Green Lucy
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.123.5
Subject(s) - offspring , medicine , endocrinology , isometric exercise , skeletal muscle , soleus muscle , weaning , contraction (grammar) , stimulation , chemistry , muscle contraction , biology , pregnancy , genetics
Maternal obesity is linked to altered offspring skeletal muscle structure and function. We tested the idea that prenatal (PRE) high fat (HF) diet would alter the deleterious effect of post‐weaning (POST) HF diet on offspring skeletal muscle contraction. C57 mice were fed a control (C, 7% kcal) or HF (45% kcal) diet in PRE and/or POST periods (C/C, C/HF, HF/C, HF/HF). Male and female 30 wk offspring (n=7‐8/group) soleus and extensor digitorum longus (EDL) muscle peak force (PF) of isometric contraction was measured in response to several electrical stimulation frequencies. In soleus and EDL, PF was greater in females than males. In female soleus (not EDL), POST HF alone markedly reduced PF (C/HF vs. C/C, P<0.01). In female soleus and EDL there was only a trend for a reduction in PF in HF/C and HF/HF groups (P=0.1). In male soleus and EDL, combined PRE and POST HF tended to reduce PF (HF/HF vs. C/C, P=0.1). In male soleus (not EDL), POST HF tended to reduce PF in PRE HF animals (HF/HF vs. HF/C, P=0.1), and PRE HF tended to reduce PF in POST HF animals (HFHF vs. C/HF, P=0.1). Thus reduced adult skeletal muscle isometric contraction force by POST HF diet is modified by a prior PRE HF diet in a sex‐ and muscle bed‐specific manner. This could reflect developmental differences in myofibre type composition or lipid deposition, and has potential implications for muscle health in adult/ageing obese individuals. Supported by The Gerald Kerkut Charitable Trust and Diabetes UK.