Premium
Black Raspberries Prevent Angiotensin II‐Induced Senescence in Vascular Smooth Muscle Cells
Author(s) -
Feresin Rafaela,
Zhao Yitong,
Salazar Gloria
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.118.5
Subject(s) - senescence , catalase , reactive oxygen species , vascular smooth muscle , polyphenol , antioxidant , angiotensin ii , oxidative stress , chemistry , biochemistry , pharmacology , endocrinology , medicine , biology , smooth muscle , receptor
The incidence of cardiovascular diseases (CVDs) is strongly associated with the physiological changes that occur with aging. Activation of the angiotensin II (Ang II) signaling pathway during aging increases the level of reactive oxygen species (ROS) leading to vascular senescence and therefore CVDs. Epidemiological studies correlate consumption of fruits and vegetables, in particular berries, with decreased incidence of CVDs, which is mainly attributed to the polyphenol content of these foods. However, the molecular mechanism mediating the protective effect of berries is not completely understood. Black raspberries (BRB) have a high polyphenol content and a powerful antioxidant capacity. Thus, the purpose of the present study was to determine the protective role of BRB polyphenol extract (PE) in Ang II‐induced senescence in vascular smooth muscle cells (VSCMs). Polyphenols were extracted from BRB using ethanol, purified by fractionation with chloroform and ethyl acetate, and total polyphenol content of crude and purified extracts was determined by Folin‐Ciocalteu method. Treatment of VSMCs with Ang II (100 nM) increased ROS levels, decreased catalase expression and increased senescence associated β‐galactosidase; thus, promoting vascular senescence. Pre‐incubation with BRBPE (200 and 400 μM) prevented Ang II‐induced increases in ROS levels and catalase downregulation leading to decreased senescence. Thus far, our findings indicate that BRBPE may enhance vascular health.