Premium
Effect of Muscle Fiber Denervation on Mitochondrial Hydrogen Peroxide Generation in Adjacent Innervated Muscle Fibres: Role in Sarcopenia?
Author(s) -
Pollock Natalie,
Vasilaki Aphrodite,
McArdle Anne,
Jackson Malcolm
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.1055.10
Subject(s) - denervation , skeletal muscle , anatomy , chemistry , muscle fibre , extensor digitorum longus muscle , biology
Nerve transection causes dramatic increases in mitochondrial generation of hydrogen peroxide (H 2 O 2 ) in the denervated skeletal muscle of rodents. We hypothesized that denervation of a limited number of fibers, such as that observed in muscles during aging, could influence H 2 O 2 generation by both denervated and innervated fibers throughout the muscle. Thy1‐YFP mice (expressing YFP in neurons) underwent full transection of the peroneal nerve or transection of only one of the 3 branches entering the anterior tibialis (AT) muscle. Following up to 10days recovery, generation of H 2 O 2 by mitochondria in permeablised fibers with intact mitochondra from different portions of the AT was assessed using Amplex Red. Results demonstrated increased H 2 O 2 generation by 3days after full transection compared with sham control, which remained elevated at 10days. In situ imaging showed loss of innervation of endplates with no evidence of post‐synaptic changes. Immuno‐fluorescent staining for markers of denervation showed neuronal cell adhesion molecule positive muscle fibers by 7days post‐surgery. Transection of the single branch of the peroneal nerve caused denervation of a portion of the AT and mitochondrial H 2 O 2 generation in the denervated portion was elevated by 3days post‐transection in a similar manner to the fully denervated muscle. Denervation of some just fibers was associated with increased mitochondrial H 2 O 2 generation in all areas of the muscle. Thus data support the hypothesis that the effect of partial denervation on muscle H 2 O 2 generation is mirrored in fully innervated fibers of the same muscle, an effect which may be mirrored in sarcopenia. Supported by the UK Biotechnology and Biological Sciences Research Council.