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L‐arginine Reduces Matrix Metalloproteinases Activity and Normalizes Oxidative Stress in Hypertensive Patients
Author(s) -
Rocha Natalia,
Garcia Vinicius,
Rocha Helena,
Cardoso Igor,
Silva Gustavo,
Barbosa Thales,
Fernandes Igor,
Secher Niels,
Vianna Lauro,
Nobrega Antonio
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.1048.2
Subject(s) - tbars , oxidative stress , nitric oxide , arginine , endocrinology , medicine , matrix metalloproteinase , thiobarbituric acid , lipid peroxidation , zymography , chemistry , biochemistry , amino acid
Defective L‐arginine–nitric oxide pathways and/or redox homeostasis are suggested mechanisms for impaired endothelium‐dependent vasodilation in hypertension. We determined effects of exogenous L‐arginine infusion on nitric oxide (NO) bioavailability, matrix metalloproteinases (MMPs) activity, and oxidative stress in hypertensive patients. Blood samples were drawn from seven hypertensive (HT; 45±5 yrs.) and eleven normotensive men (CT; 37±3 yrs.) before, during and after 30‐minute intravenous infusion of L‐arginine (30 g). Serum NO (NO analyzer), activity of MMP‐2 and MMP‐9 (gelatin zymography) were measured. Thiobarbituric acid reactive substances (TBARS) were quantified as a proxy of oxidative stress. At baseline, NO and MMPs activity were similar between groups, while TBARS were higher in the HT (P=0.03 vs. CT). During L‐arginine infusion, NO increased only in the CT (P=0.02 vs. baseline; P=0.02 vs. HT), while MMPs activity decreased in HT (P蠄0.02 vs. baseline). In HT, TBARS levels became similar to CT during L‐arginine infusion (P=0.21). L‐arginine infusion induced‐change (Δ infusion‐baseline) on NO was negatively associated with baseline systolic blood pressure (r=‐0.83, P=0.04) in HT. Thirty minutes after the infusion, NO was still higher in CT group (P=0.04 vs. HT) whereas TBARS and MMPs returned to baseline levels in HT. Despite impaired L‐arginine‐NO pathway, exogenous L‐arginine diminishes MMPs activity and normalizes oxidative stress in hypertensive patients.