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Inhibitory effects of Leishmanial sphingolipid on Endotoxin induced macrophage activation and sepsis associated inflammatory injury
Author(s) -
Das Subhadip,
Das Saha Krishna
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.1023.7
Subject(s) - sepsis , chemokine , sphingolipid , immunology , cytokine , inflammation , tlr4 , immune system , cd14 , tumor necrosis factor alpha , macrophage , proinflammatory cytokine , biology , medicine , in vitro , microbiology and biotechnology , biochemistry
Sepsis is the reflection of systemic immune response that manifests in the sequential inflammatory process in presence of infection with excessive production of inflammatory mediators and causes severe tissue injuries. Total lipid of attenuated Leishmania donovani establish as a bioactive. Moreover, sphingolipids moderate various cellular processes. So, this finding encourages us to evaluate inflammatory response in system using the microbial sphingolipid. In this study, we have used bacterial endotoxin stimulated macrophages to evaluate the efficacy of leishmanial sphingolipid (LSPL) towards the amelioration of inflammatory mediators (TNF‐α, IL‐1β, IL‐6, IL‐17, IL‐10) and transcriptional factors with associated TLR4‐CD14 expression. In vivo system, endotoxin induced murine sepsis model was used in focusing on multiple organ injury (Lungs, Liver, and Kidney) and status of different cytokine‐chemokine(s) storm. Due to the effect of LSPL, endotoxin induced inflammatory cytokine‐chemokine(s) levels were significantly reduced in serum and other major organs with the reduction of vascular permeability factors and suppressed the expression of cell adhesion molecule in septic mice evident by immunochemical approaches. These findings indicate that LSPL may prove to be a potential anti‐inflammatory agent as protection against bacterial sepsis with associated organ injury.