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Effects Of Negative Pressure Wound Therapy And Elastomeric Materials On Bone Healing
Author(s) -
Wang Rui,
Wagner William
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.1018.2
Subject(s) - wound healing , extracellular matrix , elastin , bone healing , osteoblast , biomedical engineering , chemistry , microbiology and biotechnology , alkaline phosphatase , surgery , medicine , biochemistry , pathology , in vitro , biology , enzyme
Negative pressure wound therapy has been used to accelerate clinical wound healing, however few have suggested applications for bone healing and potential pathways. In order to modulate the natural bone repair and make in vivo repair feasible, degradable materials for negative pressure wound therapy were designed. We fabricated and tested an electrospun composite of Type I collagen, chondroitin 6‐sulfate and Poly (1,8‐octanediol‐co‐citrate), termed BRM, that mimicked extracellular environment with collagen and elastin imbedded in a matrix of proteoglycans and glycoproteins. A closed chamber system was designed to generate and monitor negative pressure environments and conditions for osteoblast growth. When cultured on BRM and plastic dish with and without negative pressure, the cell proliferation was significantly greater (P<0.05) on BRM compared to electrospun collagen. With a negative pressure of ‐125mmHg, alkaline phosphatase activity was increased by Day 7 and osteoblast proliferation was increased by 1.3 folds on Day 17. The closed chamber system will be used to exam molecular pathways by the interactions of BRM and negative pressure on bone marrow derived stem cells. This study permits the evaluation of material design and feasibility of negative pressure to be explored for the accelerated bone healing.