Premium
The nuclear protein Fam60a is essential for proper her6 and ascl1b expression and thalamus development in zebrafish (LB825)
Author(s) -
Ricciardi Filomena,
Patra Chinmoy,
Engel Felix
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.lb825
Subject(s) - zebrafish , hdac1 , biology , histone , microbiology and biotechnology , gene expression , regulation of gene expression , nuclear localization sequence , gene , nuclear protein , organogenesis , genetics , histone deacetylase , transcription factor , nucleus
Vertebrate organogenesis is a highly complex and strictly orchestrated process which is dependent on the correct spatiotemporal expression of genes. Gene expression can be regulated by a variety of proteins such as histone acetyltransferases and histone deacetylases; however the conduction of these processes during organ development is still poorly understood. The small nuclear protein FAM60A has been described to be a component of the SIN3‐HDAC1 complex regulating gene expression via histone deacetylation. Here we describe the role of Fam60a during zebrafish development where its depletion leads to severe brain and heart defects. Hdac1 has been shown to promote proneural gene expression such as ascl1b and neurod4. We find that morpholino‐mediated loss of fam60a causes expanded her6 and reduced ascl1b expression in the zebrafish brain. Furthermore, we show that FAM60A possesses a functional Nuclear Localization Signal (NLS) which is essential for its correct function in brain development. Grant Funding Source : Universitätsklinikum Erlangen