Premium
Nucleoprotein supplementation combined with intermittent loading prevents capillary regression induced by hindlimb unloading in rat soleus muscle (LB810)
Author(s) -
Hirayama Yusuke,
Nakanishi Ryosuke,
Maeshige Noriaki,
Fujino Hidemi
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.lb810
Subject(s) - hindlimb , angiogenesis , soleus muscle , endocrinology , chemistry , medicine , nucleoprotein , skeletal muscle , biochemistry , gene
Amino acids supplementation and exercise have been shown to promote physical activity‐induced angiogenesis. Therefore, the aim of this study was to determine the effects of nucleoprotein, which was composed of wide variety of amino acids, supplementation combined with intermittent loading on the capillary regression. Hindlimb unloading resulted in decrease of capillary‐to‐fiber (C/F) ratio and citrate synthase (CS) activity in the soleus muscle. Intermittent loading could not prevent the decrease in C/F ratio and CS activity, whereas nucleoprotein supplementation combined with intermittent loading could prevent the decrease in C/F ratio and CS activity in the soleus muscle. In addition, disuse‐associated changes in kinase insert domain receptor (KDR), a pro‐angiogenesis factor, and thrombospondin‐1 (TSP‐1), an anti‐angiogenesis factor, were prevented by nucleoprotein supplementation combined with intermittent loading. These results indicate that nucleoprotein supplementation combined with intermittent loading is effective to prevent capillary regression, maintaining oxidative enzyme activity and the balance between pro‐ and anti‐angiogenesis factors. Supported by Grants‐in‐Aid for Science Research from the Japanese Ministry of Education, Culture, Sports, Science and Technology, and the internal funds of Nissan Chemical Industries, Ltd.