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Chronic intermittent hypoxia augments bleomycin induced lung fibrosis in rats (LB794)
Author(s) -
Braun Rudolf,
Broytman Oleg,
Pegelow David,
Hsu PeiNing,
Mei Linda,
Modi Dhruvangkumar,
Eldridge Marlowe,
Teodorescu Mihaela
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.lb794
Subject(s) - medicine , bleomycin , lung , pulmonary fibrosis , intermittent hypoxia , fibrosis , hypoxia (environmental) , pulmonary function testing , gastroenterology , saline , anesthesia , obstructive sleep apnea , chemistry , chemotherapy , organic chemistry , oxygen
Rationale: Obstructive sleep apnea (OSA) is common among patients with idiopatic pulmonary fibrosis (IPF), where it has been linked with increased mortality. Chronic intemittent hypoxia (CIH) _ its hallmark feature _ may be one culprit. We tested the effects of CIH exposure on lung collagen, gas exchange and lung function following intratracheal bleomycin instillation in rats. Methods: Four groups (n=4/group) of Sprague Dawley rats were instilled with bleomycin (1.5 U/kg) (BLEO) or saline (SAL). Five days after instillation, rats were subjected to CIH (10% FiO 2 , 30 episodes/h, 10h/day) vs. normoxia (NORM) for 30 days. Total collagen in the right lung, pO 2 and pCO 2 in the exhaled air, and lung function by body plethysmography were compared between groups. Results: CIH resulted in: 1) significant increase in total collagen in bleomycin injured lungs compared to BLEO/NORM or the SAL controls ( Figure 1 ); 2) significant exhaled O 2 abnormalities in both SAL and BLEO treated groups compared to the respective NORM groups ( Figure 2 ); 3) a trend of reduced FEV0.1/FVC ratio in CIH treated groups in both the BLEO and SAL compared to the NORM group (0.78±0.10 vs. 0.71±0.14 and 0.67±0.17 vs. 0.61±0.15). C onclusions: CIH aggravates lung fibrosis and gas exchange abnormalities following bleomycin. These findings underscore the potential of OSA to exacerbate IPF.Grant Funding Source : UW‐Madison Department of Medicine Pilot Funding