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Hypoxic and hypercapnic ventilatory response in rats with estradiol valerate‐induced polycystic ovaries (LB773)
Author(s) -
Montrezor Luís,
Carvalho Débora,
AnselmoFranci Janete,
Bícego Kênia,
Gargaglioni Luciane
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.lb773
Subject(s) - estradiol valerate , hypercapnia , polycystic ovary , endocrinology , medicine , ventilation (architecture) , hypoxia (environmental) , chemistry , testosterone propionate , testosterone (patch) , zoology , hormone , respiratory system , biology , androgen , oxygen , obesity , mechanical engineering , insulin resistance , organic chemistry , engineering
We evaluated pulmonary ventilation (VE) and body temperature (Tb) during hypercapnia (7%CO2) and hypoxia (7%O2) in a female rat model in which estradiol valerate (EV) (2mg/0.2mL/rat or oil (control) were administered intramuscularly in a single dose to induce Polycystic ovary syndrome (PCOS). The experiments were performed 30, 45 or 60 days after EV injection. The ovaries of 30, 45 and 60 days EV group displayed typical PCOS‐like changes. Plasma levels of estradiol (E2) and progesterone (P4) increased in 30 (E2: 74.5 ± 23.5 pg/mL; P4: 18.3 ±4.5 ng/mL), 45 (E2: 70.8 ± 17.5 pg/mL; P4: 21.3 ±7.6 ng/mL) and 60 (E2: 71.4 ± 4.7 pg/mL; P4: 23.7 ±4.5 ng/mL) days EV groups whereas testosterone (T) increased only in 45 (58.4±6.5 pg/mL) and 60 (79.5±18.3 pg/mL) days EV groups compared with control group (E2: 46.9±11.3 pg/mL; P4: 9.9±3.4 ng/mL; T: 22.6±1.9 pg/mL). 30, 45, 60 EV treatment did not affect resting ventilation and hypoxic ventilatory response. However, the increase in ventilation produced by hypercapnia was attenuated after 30 days EV group (1978.9 ± 85.7 mL.kg‐1.min‐1 in control group, 1651.2 ± 369.3 in 45 days group and 1617.4± 131.4 in 60 days group vs 771.0 ± 172.4 in 30 days group). Hypoxia induced Tb to reduce in all groups, a response that was amplified in 30 days EV group. Our results suggest that initial alterations promoted by EV treatment changes CO2‐drive to breathe and hypoxia‐induced drop in Tb, and these responses are compensated in 45 and 60 days EV females.