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Low salt intake during pregnancy alters glucose metabolism and DNA methylation in the offspring (LB765)
Author(s) -
Siqueira Flavia,
Oliveira Ivone,
Furukawa Luzia,
Heimann Joel
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.lb765
Subject(s) - offspring , endocrinology , medicine , pregnancy , gestation , insulin resistance , insulin , biology , dna methylation , gene expression , gene , biochemistry , genetics
Maternal nutritional alterations during pregnancy are associated with metabolic disorders and the period of pregnancy in which these alterations influence adult offspring remains uncertain. Epigenetic changes are proposed to underlie these disorders. Methods: Twelve‐week‐old female Wistar rats were fed a low‐salt (LS) or normal‐salt (NS) diet during gestation or LS during the first (LS10) or second (LS20) half of gestation. Body weight, blood glucose, insulin (ITT) and glucose (GTT) tolerance tests, HOMA‐IR, gene expression and DNA methylation were mapped in the male offspring. Birth weight was lower in LS20 and LS rats compared with NS and LS10 rats. HOMA‐IR was higher in 12‐week‐old LS compared with NS rats and in 20‐week‐old LS10 compared with NS rats. Serum insulin was higher in 20‐week‐old LS10 rats compared with NS rats. The GTT indicated glucose intolerance only in 12‐ and 20‐week‐old LS rats. Methylation of the Insr, Igf1, Igf1r, Ins1 and Ins2 genes in liver of neonates and in liver, white adipose tissue and muscle in 20‐week‐old offspring were influenced by low‐salt intake during pregnancy. In conclusion low‐salt diet in the second half of pregnancy can result in low birth weights in the offspring. Glucose intolerance observed in adult offspring occurred only if low salt intake was given throughout pregnancy. However, insulin resistance in response to low salt intake during pregnancy is related to the time at which this insult occurs and to the age of the offspring. Alterations in the DNA methylation of Igf1 were observed to be correlated with low birth weight in response to low salt feeding during pregnancy. Grant Funding Source : Supported by FAPESP and CAPES