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Brilliant Blue G attenuates the effects of intestinal ischemia/reperfusion injury in rats (LB747)
Author(s) -
Palombit Kelly,
Mendes Cristina,
Castelucci Patricia
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.lb747
Subject(s) - reperfusion injury , chemistry , myeloperoxidase , nitric oxide synthase , antagonist , ischemia , extracellular , endocrinology , staining , medicine , receptor , pathology , nitric oxide , inflammation , biochemistry
During pathological conditions there is an increased release ATP into the extracellular space, activating the P2X7 receptor. Several studies have shown that injury can be attenuated by the antagonist of this receptor, the Brilliant Blue G (BBG). In the present work we analyzed the effects of intestinal ischemia and reperfusion (I/R) and the effects of the BBG on rats ileum. Tissues were obtained from male Wistar rats (n=5). The ileal artery was occluded for 45 minutes with an atraumatic vascular clamp. BBG (50 and 100 mg/kg) or saline (vehicle) was given subcutaneous 1 h after injury (24 h group). The sham group was rats without the arterial occlusion. We analyzed also the I/R 0 h group (not reperfusion). Myenteric neurons were evaluated for immunoreactivity (ir) against the P2X7 receptor, nitric oxide synthase (NOS), neurofilament (NF), choline acetyl transferase (ChAT) and anti‐HuC/D (Hu). Technique histology staining to hematoxylin and eosin (HE) was performed to evaluate the integrity of the intestinal wall and myeloperoxidase technique (MPO) to verify the marking of neutrophils. Our qualitative results showed that I/R caused histological damage to the mucosa and muscle and infiltration of neutrophils into the muscle layers. Our quantitative results showed that there was a decrease in density (neurons/cm2) P2X7‐ir, NOS‐ir, NF‐ir, ChAT‐ir and Hu‐ir neurons in I/R 0 h and 24 h groups. In I/R groups where the antagonist BBG was injected, the neuronal density was less affected. Analysis in the area of the cell body profile different classes showed generally decreases in the profile area in the I/R groups and recovery profile area of the I/R BBG‐50 and BBG‐100 groups. This work indicates that deleterious effects are greater in groups subjected to I/R in rats without application BBG, suggesting that BBG has protective effects on the intestinal wall and in different neuronal classes. Grant Funding Source : FAPESP