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Expression of multiple adenylyl cyclase isoforms in pancreatic cancer cell line (LB740)
Author(s) -
QUINN SIERRA,
Sohail Syeda,
SABBATINI MARIA
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.lb740
Subject(s) - gene isoform , pancreatic cancer , adenylyl cyclase , transmembrane protein , biology , cancer research , cell culture , adenocarcinoma , signal transduction , cancer , microbiology and biotechnology , gene , biochemistry , genetics , receptor
Cyclic AMP (cAMP) is implicated in the proliferation and differentiation of several normal and malignant cell types, including pancreatic cancer. Adenylyl Cyclases (ACs) are enzymes which increase the levels of cAMP when activated. Nine transmembrane AC isoforms are known, and each one has its own pattern of expression and regulation. Objective: In this study we establish which transmembrane AC isoforms are expressed in the pancreatic adenocarcinoma epithelial cell line HPAC. Results: Using RT‐PCR we found five different isoforms: AC1, AC3, AC6, AC7 and AC9. The expression profile is similar to that in pancreatic duct cells, but AC1, which is only expressed in pancreatic cancer cells. Future goals: Study which isoform is involved in the development and progression of HPAC cells. Discussion: Overall, the pathophysiological significance of each AC isoform will provide an important insight into the cellular signaling in pancreatic cancer.