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Skeletal muscle TLR4 expression is associated with body composition, but not physical activity level in older adults (LB74)
Author(s) -
Connors Ian,
Mott Rachael,
Trivedi Rudra,
Combs Larissa,
Heilbronn Chase,
Timmerman Kyle
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.lb74
Subject(s) - skeletal muscle , medicine , endocrinology , inflammation , tlr4 , vastus lateralis muscle
Elevated skeletal muscle expression of toll‐like receptor 4 (TLR4) has been linked to increased inflammation in clinical populations. Higher physical activity level (PA) is associated with decreased disease risk and inflammation. The purpose of this study was to examine the relationships among PA, body composition, and skeletal muscle TLR4 in older adults. Methods: In 23 subjects (age=67±4y, BMI=26±3 kg•m ‐2 ) self‐reported PA (kilocalories•week ‐1 ), estimated maximal oxygen consumption (VO 2 max), and body composition (air plethysmography) were measured. TLR4 was measured in skeletal muscle biopsies ( vastus lateralis ) using western blot analyses. Pearson product‐moment correlations were run between variables. Significance was set to p<0.05. Results: There was no significant correlation between TLR4 and PA. There was a significant correlation between %body fat and skeletal muscle TLR4 (r=0.496, p<0.05). There was also a significant negative correlation between VO 2 max and BF% (r=‐0.45, p<0.05). Conclusion: These preliminary data do not show an association between PA and TLR4 in skeletal muscle from healthy older adults. However, a positive correlation was found between skeletal muscle TLR4 and % body fat. Thus, elevated skeletal muscle expression of TLR4 may underlie reports indicating that increased adiposity is associated with elevated inflammation and disease risk.