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Localization of Urea Transporters UT‐A1 and UT‐B in Human Eccrine Clear Cell (LB719)
Author(s) -
Keller Raymond,
Sands Jeff
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.lb719
Subject(s) - eccrine sweat gland , sweat , sweat gland , endocrinology , immunoperoxidase , medicine , human skin , urea , immunohistochemistry , kidney , biology , excretion , staining , chemistry , antibody , biochemistry , monoclonal antibody , immunology , genetics
Urea, a solute classically excreted by the kidney that accumulates in kidney failure, is known to be concentrated in human sweat. Urea is also excreted in the sweat of rat eccrine footpad glands. Therefore, sweat represents an alternative route of excretion for urea. The purpose of this study was to probe for a transporter that might contribute to urea excretion in human skin and rat footpad exocrine glands. Immunohistochemistry was performed on 5 human skin and 5 Sprague‐Dawley rat footpad samples. Human skin was formalin fixed and paraffin embedded for sectioning at 5μm while rat samples were formalin fixed and cryogenically sectioned at 5μm. Polyclonal COOH‐terminal UT‐A and UT‐B antibodies were used to identify the location of UT‐A1 and UT‐B, respectively. Human and rat specimens each contained hair follicles, sebaceous glands, and eccrine glands. Immunoperoxidase staining showed that UT‐B is expressed in the plasma membrane of clear cells in the human eccrine sweat gland. The antibodies did not detect urea transporters in any rat footpad epidermal structures nor the human hair follicles or sebaceous glands. In conclusion, these results suggest the presence of UT‐B in the human eccrine gland. This suggests that UT‐B may contribute to urea secretion in sweat. Future studies are needed to determine whether uremia alters the expression of UT‐B in eccrine sweat glands.舃