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Sodium Nitrite Supplementation Alters the Human Metabolome in Healthy Middle‐Aged and Older Adults (LB703)
Author(s) -
Johnson Lawrence,
Picciotto Natalie,
CruickshankQuinn Charmion,
Reisdorph Nichole,
Giordano Tony,
DeVan Allison,
Seals Douglas,
Sindler Amy
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.lb703
Subject(s) - metabolome , metabolomics , nitrite , nitric oxide , metabolite , chemistry , sphingolipid , bioavailability , metabolism , lipidomics , biochemistry , pharmacology , endocrinology , medicine , biology , chromatography , organic chemistry , nitrate
Reduced nitric oxide (NO) bioavailability contributes to physiological dysfunction with aging. Oral sodium nitrite supplementation (SN) increases circulating nitrite, a precursor of NO, and improves some physiological functions in middle‐aged and older (MA/O) adults. To gain insight into the underlying molecular mechanisms, the metabolome was assessed by liquid chromatography‐mass spectrometry in plasma samples from MA/O adults before and after SN (80 mg/d capsules, TheraVasc, Inc.) (n=8, 59±2 yrs) or placebo (P, n=7, 61±3 yrs) (randomized, double blind). Of ~4,000 metabolites detected, significant changes in 37 intermediary metabolites were observed in SN vs. P groups (minimum 1.5 fold‐change, p<0.05). Several metabolites associated with a pro‐aging phenotype changed with SN, including pro‐inflammatory mediators and molecules related to sphingolipid, glycan, glycerophospholipid, fatty acyl, polyketide, and choline metabolism. Importantly, these SN‐induced changes occurred in the absence of changes in clinical characteristics including body composition and blood lipid profiles (all p>0.05). These preliminary results suggest that SN influences human plasma metabolome pathways linked to aging. Further targeted analysis will allow the identification of novel biomarkers in metabolic pathways modulated by SN and offer insight into the mechanisms by which SN improves specific domains of physiological function in MA/O adults. Grant Funding Source : Supported by NIH HL107105, RR025780, AG000279