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Increased β‐adrenergic inotropy in ventricular myocardium from trpm4‐/‐ mice. (LB666)
Author(s) -
Mathar Ilka,
Kecskes Miklos,
Van der Mieren Gerry,
Jacobs Griet,
Camacho Londoño Juan E.,
Uhl Sebastian,
Flockerzi Veit,
Voets Thomas,
Freichel Marc,
Nilius Bernd,
Herijgers Paul,
Vennekens Rudi
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.lb666
Subject(s) - contractility , inotrope , myocyte , ventricular action potential , medicine , chemistry , cardiac transient outward potassium current , endocrinology , patch clamp , cardiac muscle , in vivo , repolarization , cardiac function curve , electrophysiology , cardiology , heart failure , biology , microbiology and biotechnology
RATIONALE: The Trpm4 gene has recently been associated with several disorders, including cardiac conduction diseases and Brugada syndrome. Transient receptor potential member 4 (TRPM4) proteins constitute Ca(2+)‐activated, but Ca(2+)‐impermeable, nonselective cation channels and are expressed both in atrial and in ventricular cardiomyocytes. The physiological function of TRPM4 in the heart remains, however, incompletely understood. OBJECTIVE: To establish the role of TRPM4 in cardiac muscle function. METHODS AND RESULTS: We used TRPM4 knockout mice and performed patch‐clamp experiments, membrane potential measurements, microfluorometry, contractility measurements, and in vivo pressure‐volume loop analysis. We demonstrate that TRPM4 proteins are functionally present in mouse ventricular myocytes and are activated on Ca(2+)‐induced Ca(2+) release. In Trpm4(‐/‐) mice, cardiac muscle displays an increased β‐adrenergic inotropic response both in vitro and in vivo. Measurements of action potential duration show a significantly decreased time for 50% and 90% repolarization in Trpm4(‐/‐) ventricular myocytes. We provide evidence that this change in action potential shape leads to an increased driving force for the L‐type Ca(2+) current during the action potential, which explains the altered contractility of the heart muscle. CONCLUSIONS: Our results show that functional TRPM4 proteins are novel determinants of the inotropic effect of β‐adrenergic stimulation on the ventricular heart muscle.