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Effect of nanoparticles on relaxation of small mesenteric arteries of spontaneously hypertensive rats (LB659)
Author(s) -
Albarwani Sulayma,
AlLawati Intisar,
AlSiyabi Sultan,
Tanira Musbah
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.lb659
Subject(s) - myograph , mesenteric arteries , sodium nitroprusside , chemistry , vasodilation , saline , medicine , endocrinology , acetylcholine , relaxation (psychology) , endothelium , ketamine , artery , anesthesia , nitric oxide
Hypertension causes impairment of endothelium‐dependent relaxation. There is evidence that nanoparticles may affect vascular relaxation. This study investigated effect of silica nanoparticles (50nm; SiNP) on EDR and independent relaxations of small mesenteric arteries of spontaneously hypertensive rats. Rats (4 month) were divided into 6 groups, each injected intravenously with one dose of SiNP (0.5, 1.0, 10, 25.0 and 50mg/Kg; treated) or normal saline (control). After 24 hours, rats were sacrificed with an overdose of a mixture of ketamine (140 mg kg−1 I.M.) and xylazine (40 mg kg−1 I.M.). Third order mesenteric arteries were dissected out and mounted on wire myograph; tension was recorded. Arteries were sub‐maximally pre‐contracted with phenyephrine (PE; 4µM) and then relaxed in a cumulative concentration‐response manner with acetylcholine (Ach 0.1‐10µM) or sodium nitroprusside (SNP; 0.1‐10 µM). Relaxations induced by Ach were not significantly different in arteries obtained from treated compared to control animals. Whereas, concentration‐response curves to SNP were shifted to the left in arteries isolated from rats injected with 25 and 50 mg/Kg. IC50s were (nM mean ± s.e.m); 94±6.6, 48.3±9.3, 52.3 ±10.1 for control, 25 and 50 mg/Kg respectively (p=0.05). It is concluded that SiNP administration affected endothelium‐independent but not endothelium‐dependent relaxation.