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Protective effects of dipotassium glycyrrhizinate against snake venom muscle damage (LB633)
Author(s) -
Ziegler Jussara,
Gracia Marta,
Oliveira Thais,
Nascimento Laís,
Rocha Thalita
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.lb633
Subject(s) - bothrops , venom , myotoxin , myofibril , myocyte , myod , sarcoplasm , sarcolemma , pharmacology , snake venom , inflammation , chemistry , medicine , myogenesis , endocrinology , andrology , biochemistry , endoplasmic reticulum
Myonecrosis is a serious consequence of Bothrops snake bites that may be due to destabilization and rupture of sarcolemma and organelles, inflammation, leading to permanent loss of muscle. Antivenom has been ineffective to neutralize this tissue damage and plant extracts have being studied as an associative therapy. In this research we analyzed the protective effect of Dipotassium Glycyrrhizinate (DPG) against myotoxic effects of Bothrops jararacussu snake venom using in vivo experimental protocols. DPG, isolated from Glicirriza glabra, is an anti‐inflammatory agent employed in treating dermatitis. The myotoxic process was followed in male Swiss mice gastrocnemius muscle at early (3 hours), intermediate (24 hours), late (3‐7 days) and final (21 days) phases after intramuscular injection of B. jararacussu snake venom (100µg/µL), followed by 2% DPG injection 30 minutes later. Control muscles were injected with DPG only. Muscles sections (10µm) were processed for light microscopy and immunohistochemistry for TNFα, IFNγ, myoD and miogenin. At early and intermediate phases, the muscle damage was characterized by delta lesions, densely‐clumped myofibrils and empty‐looking sarcoplasmic areas, inflammatory infiltrate and intense TNFα and IFNγ expression. Satellite cells and myoblasts, followed by myotubes were observed, from late to final phases, by myoD and miogenin expression. Cytokines expression coincided with the intense muscle proteolysis caused by the venom and the climax of regeneration, whereas cytokines decline, corresponded to periods of tissue remodeling and intense myofibril synthesis. The results show that DPG was able to decrease the myotoxic effects of this venom by inducing the inflammatory response and muscle regeneration. Grant Funding Source : Supported by FAPESP 2013/10163‐4