Premium
The combination of curcumin and ginkgolide B reduces cyst growth by multiple cell signaling pathways (LB586)
Author(s) -
Gao Jinsheng,
Li Yousong,
Wang Xixing,
Hao Xuliang,
Liu Likun,
Zhou Hong,
Yang Baoxue
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.lb586
Subject(s) - curcumin , cyst , polycystic kidney disease , pharmacology , autosomal dominant polycystic kidney disease , biology , kidney , medicine , pathology
Autosomal dominant polycystic kidney disease (ADPKD), a common disease with high incidence ratio, often causes great harm to people. However, no effective drug is currently available. The purpose of this study was to evaluate the cyst coinhibition of curcumin and ginkgolide B and to elucidate their different inhibition mechanisms. In vitro MDCK cyst model was used to observe the coeffect of curcumin and ginkgolide B on cyst development. The results showed that the shapes of cysts were different after curcumin or ginkgolide B treatment. The cysts treated with curcumin often became small thick‐walled cysts, the cysts exposed to ginkgolide B often turned into small thin‐walled cysts, and the cysts after coinhibition of curcumin and ginkgolide B often became round shaped colonies, which suggested the combination of two drugs was more effective than any of them. Our study and foreign study have confirmed the cyst inhibition effect of curcumin and ginkgolide B on polycystic kidney disease mice. Curcumin may execute its anti‐cyst activity partly by blocking MAPK signaling pathway and m‐TOR signaling pathway. Furthermore, our study demonstrated that curcumin up‐regulated Ca 2+ level in MDCK cell, which may be related to its cyst inhibition. Ginkgolide B may execute its anti‐cyst activity partly by blocking MAPK signaling pathway. The results provide proof‐of‐concept for application of combination of curcumin and ginkgolide B to reduce cyst growth in polycystic kidney disease. Grant Funding Source : The National Youth Science Fund (81302828), Shanxi Province Youth Science and Technology Research Fund (2013021037‐3), Taiyuan Science and Technology Research Fund (201110‐1555), Shanxi Province Youth Science and Technology Research Fund (201201040)