Premium
Peanut butter increases kale β‐carotene absorption and conversion to vitamin A in pre‐school children (LB417)
Author(s) -
Muzhingi Tawanda,
Tang Guangwen,
Yeum KyungJin,
Bermudez Odilia,
Siwela Andrew
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.lb417
Subject(s) - vitamin , carotene , retinol , brassica oleracea , chemistry , retinyl acetate , peanut oil , food science , carotenoid , zoology , canola , vitamin a deficiency , biochemistry , biology , botany , raw material , organic chemistry
Brassica oleracea var. acephala (kale) is a rich source of β‐carotene that can be used to provide vitamin A to vitamin A deficient preschool children in Africa. This study was designed to determine the effect of peanut butter on the vitamin A equivalence and bioconversion efficiency of kale β‐carotene to vitamin A in preschool children. Deuterium labeled kale was grown in a hydroponic medium with 23 atom% 2H2O during grain development. The kale β‐carotene showed the highest abundance of enrichment as [2H9] β‐carotene. Preschool children (n = 37; age 12‐36 mo) were randomly assigned to consume 50g cooked kale (1.5mg β‐carotene content) with either peanut butter (PBG) or with lard (LG). On day 1 after a baseline blood draw; all subjects consumed the labeled kale doses and 1 mg [13C10] retinyl acetate capsule. Blood samples were collected from five subjects per‐time point per group over 21 d. Serum samples processed were analyzed by using NCI‐GC/MS for the enrichments of labeled [2H]retinol from kale from [2H9]β‐carotene and from [13C10]retinol from reference dose. The area under the curve (AUCs) of molar enrichment at days 1, 2, 3, 6, 15, and 21 after the labeled doses was 21.1 and 27.9 (nmole) for [2H] retinol from LG and PBG kale [2H9] β‐carotene respectively. The AUC of [13C10] retinol from was 223.4 (LG) and 314.45 (nmol) (PBG) respectively. The calculated conversion factors were 19.0 and 19.3 to 1 by weight for LG and PBG respectively. Peanut butter had higher β‐carotene absorption response than the lard group. This study showed that kale β‐carotene is efficiently absorbed and converted to vitamin A in the presence of fat in children. Therefore, kale should be promoted as a complementary food for children in developing countries who are vulnerable to VAD and general malnutrition. Grant Funding Source : IAEA