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Comparison of extract and flavonoid‐rich Fraction from mulberry on proliferation and lipid accumulation in 3T3‐L1 adipocytes (LB392)
Author(s) -
Kim GunHee,
Park Hee Sook
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.lb392
Subject(s) - flavonoid , traditional medicine , adipocyte , chemistry , pharmacology , biology , biochemistry , medicine , antioxidant , adipose tissue
This study was performed to investigated the effect of ethanol extracts and fraction of Morus alba L. on adipocyte differentiation in 3T3‐L1 cell. Obesity is major obstacle for improving health. Therefore, the therapeutic approach using natural plants has been proposed as a novel strategy for preventing obesity. Fruits of mulberry (Morus alba L.) belonging to the Moraceae family have been widely used for therapeutic purposes in Asian countries for centuries. Many study investigated that mulberry fruits can be used as a warming agent, as a remedy for dysentery, and as a tonic, sedative, laxative, odontalgic, anthelmintic, expectorant, and emetic. This study was performed to investigated the effect of ethanol extract and flavonoid‐rich fraction (A2) of Morus alba. on adipocyte differentiation in 3T3‐L1cell. The anti‐proliferation effects of ethanolic extracts and A2 fraction were measured by MTT assay. Cell viability of ehtanolic extract decreased but not A2 fraction during 72 hr in preadipocyte. For anti‐adipogenic effects of Morus alba, ethanolic extract and A2 fraction were examined Oil red O staining assay. Treatment of 3T3‐L1 cells with ethanolic extracts and A2 fraction decreased adipocyte differentiation in a dose‐dependent manner by 18.1% and 32.7% in 100 μg/ml, respectively. The results indicate that Morus alba L. is a possible candidate for regulating lipid accumulation in obesity. Grant Funding Source : This work was supported by Priority Research Centers Program through the National Research Foundation of Korea(NRF) funded by the Ministry of Education, Science and Technology(KNF‐2009‐0094017).