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Effects of lycopene supplementation on oxidative stress markers in adipose tissue of obese rats (LB389)
Author(s) -
Luvizotto Renata,
Nascimento André,
Wang XiangDong,
Yeum KyungJin,
Ferreira Ana Lúcia
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.lb389
Subject(s) - lycopene , adipose tissue , oxidative stress , nadph oxidase , medicine , protein carbonylation , endocrinology , chemistry , antioxidant , lipid peroxidation , oxidase test , biochemistry , enzyme
Background : Obesity has been associated with oxidative stress, and NADPH oxidase system is known as main source of reactive species. Lycopene is a potent antioxidant. However, it is not known the influence of lycopene on NADPH‐oxidase activity in adipose tissue of obese rats. Objective : To analyze the effects of lycopene supplementation on oxidative stress markers, including NADPH‐oxidase activity, in adipose tissue of diet‐induced obese rats. Methods : Male Wistar rats were first fed with hypercaloric diet (HD, n=14) for 6 weeks and afterwards, these rats were randomly assigned to receive HD (n=7) or HD with lycopene (10 mg/Kg BW/d; HD+L, n=7) by gavage for additional 6 weeks. Adipose tissue was collected for evaluation of NADPH‐oxidase activity and other oxidative stress markers, by HPLC system and immunoassay, respectively. Results : In obesity, lycopene treatment for six weeks decreased protein carbonylation (HD: 5.24 ± 0.77 vs HD+L: 3.78 ± 0.50) in visceral adipose tissue, and 4‐HNE in visceral (HD: 0.43 ± 0.04 vs HD+L: 0.36 ± 0.02) and retroperitoneal (HD: 0.40 ± 0.07 vs HD+L: 0,31 ± 0,02) adipose tissue. Absence of difference in NADPH oxidase activity was found between HD and HD+L groups. Conclusion : Lycopene supplementation decreased lipid peroxidation and protein carbonylation and did not interfere in NADPD‐oxidase activity in adipose tissue. These data suggest that lycopene may be an effective strategy in reducing some oxidative stress markers in obesity. Grant Funding Source : Supported by FAPESP #10/06100‐9 and #10/19746‐4