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Sinigrin Suppresses Adipocyte Differentiation through the MAPKs Pathway in 3T3‐L1 Preadipocytes (LB362)
Author(s) -
Jang Yeon jeong,
Lee Heewoen
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.lb362
Subject(s) - sinigrin , adipocyte , mapk/erk pathway , transcription factor , 3t3 l1 , p38 mitogen activated protein kinases , chemistry , microbiology and biotechnology , biology , glucosinolate , medicine , biochemistry , phosphorylation , endocrinology , brassica , botany , adipose tissue , gene
Obesity is a major risk factor for various diseases such as type 2 diabetes, hypertension, cardiovascular diseases and certain types of cancer. In these days, anti‐obesity effects of natural products are of intense medical interest. In the present study, we examined the inhibitory effects of sinigrin, belongs to the family of glucosides found in some plants of the Brassicaceae family such as Brussels sprouts, broccoli, and the seeds of black mustard ( Brassica nigra ), on adipocyte differentiation in 3T3‐L1 cells. Sinigrin attenuated the accumulation of lipid droplets stained with Oil Red O. The expression of peroxisome proliferator‐activated receptor γ (PPARγ), a main transcription factor for adipocyte differentiation, was dose dependently decreased by sinigrin treatment. Also, the effects of sinigrin on the expression of CCAAT/enhancer‐binding protein β (C/EBPβ) and MAPK activation were investigated. The results revealed that sinigrin sequentially inhibited the phosphorylation of ERK, p38 and JNK. Moreover, sinigrin decreased the phosphorylation of C/EBPβ at Thr 188 , an essential step for transcriptional activation of C/EBPβ. Our results indicate that the inhibition of adipocyte differentiaion by sinigrin may be related to the regulation of the MAPKs pathway. Thus, sinigrin could be a novel therapeutic candidate for regulating adipocyte differentiation.