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Metalloproteomic approach of mercury in breast milk samples of lactating women in the Amazon region, Brazil. (LB254)
Author(s) -
Queiroz João,
Santos Felipe,
Braga Camila,
Leite Aline,
Buzalaf Marília,
Zara Luiz,
Okoshi Marina,
Padilha Cilene do,
Padilha Pedro de
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.lb254
Subject(s) - mercury (programming language) , spots , chemistry , fractionation , cadmium , breast milk , mass spectrometry , proteome , analytical chemistry (journal) , chromatography , biochemistry , organic chemistry , computer science , programming language
The aim of this study is to present the metalloproteomic results obtained for the protein fractionation in breast milk samples of lactating women from JIRAU Hydroelectric Power Plant ‐ Madeira River/Amazon region, Brazil. The protein spots obtained after the fractionation by 2D‐PAGE were cut from the gel and analyzed by Thermal Decomposition and Amalgamation Coupled with Atomic Absorption and the protein spots, in which were detected mercury, were characterized by mass spectrometry (ESI‐MS/MS). The mercury determinations indicated that this element is linked in two protein spots with molar mass (Mm) of 10.80 (spot 1) and 15.00 kDa (spot 2) and with concentration of 4.70 and 12.10 mg of mercury per gram of protein in the protein spots, respectively. Based on these concentrations was possible to estimate one mercury atom per four protein molecules in relation to the spot 1 and one mercury atom per protein molecule in relation to the spot 2. The ESI‐MS/MS analysis allowed to characterize the spot 1 as a fragment of beta‐casein (Mm = 26.00 kDa) and the spot 2 of lysozyme (Mm = 17.70 kDa). Grant Funding Source : Financial Support: ESBR PD‐6631‐0001/2012 and Fapesp