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β‐like Importins mediate the nuclear translocation of JNK1/2 and p38α/β (LB219)
Author(s) -
Zehorai Eldar,
Seger Rony
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.lb219
Subject(s) - importin , nuclear transport , microbiology and biotechnology , chromosomal translocation , nuclear localization sequence , transcription factor , p38 mitogen activated protein kinases , chemistry , cell nucleus , nls , phosphorylation , biology , nucleus , mapk/erk pathway , gene , biochemistry
The rapid nuclear translocation of signaling proteins upon stimulation plays an important role in the regulation of de‐novo gene expression. We have studied the stimulated nuclear shuttling of JNK and p38 MAPKs, and found that they translocate into the nucleus in NLS‐ or NTS‐independent manners, unrelated to their catalytic activity. In addition, we found that this translocation is mediated by three β‐like importins (Imp 3, 7 and 9). Knocking down these importins inhibits the stimulated nuclear translocation of the MAPKs, and thereby, activation of their transcription factors targets. We demonstrated that this process involves the binding of either JNK1/2 or p38α/β to Imp7 or Imp9 and requires their further association with Imp3. These heterotrimers then translocate to the nuclear envelope, where Imp3 remains, while Imp7 or Imp9 escort the MAPKs through the nuclear pores. These results suggest that β‐like importins are central mediators of stimulated nuclear translocation of signaling proteins, and therefore add a central level of regulation to stimulated transcription.