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Histological changes in the structure of lateral and medial menisci following the destabilization of the knee in a rat model (LB18)
Author(s) -
Riccardi Margaret,
Fraticelli Nicole,
Jordan Rebecca,
Boehm Karl,
Ginsberg Zachary,
Marberry Kevin,
Kondrashov Peter
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.lb18
Subject(s) - fibrocartilage , medial meniscus , meniscus , anatomy , hyaline cartilage , cartilage , hyaline , h&e stain , lateral meniscus , medicine , tibia , connective tissue , pathology , staining , articular cartilage , osteoarthritis , anterior cruciate ligament , physics , alternative medicine , incidence (geometry) , optics
Three month‐old rats underwent destabilization of the medial meniscus (DMM) surgery, to replicate meniscal damage, or sham surgery as a control, and were sacrificed 8 weeks later. Knees were fixed in formalin, decalcified, embedded in paraffin, and sectioned coronally at 12 µm. Sections were stained with hematoxylin and eosin and scored by 2 investigators using point counting with a grid probe. Based on existing data, each square was scored as one of the 4 tissue types normally present in the meniscus: dense connective tissue, fibrocartilage, hyaline cartilage, or bone. Percentages of these tissue types were used to quantify the histological composition of the menisci. Preliminary data on the histological composition of the lateral and medial menisci showed a significant difference between the two. In the control group, the lateral meniscus contained significantly more hyaline cartilage than the medial. In the DMM group, the lateral meniscus contained more bone and hyaline cartilage than the medial. The comparison of the histological composition of the menisci between the DMM and control groups revealed a statistically significant increase in the percentage of bone in the lateral meniscus in the DMM group. The changes in the architecture of the knee following the destabilization of the medial meniscus in the rat model resulted in the increased ossification of the lateral meniscus compared to the control.