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New immunological assays for diagnosis of Schistosoma mansoni for clinical acute and/or chronic forms (990.1)
Author(s) -
Grenfell Queiroz Rafaella,
SilvaMoraes Vanessa,
Harn Donald,
Coelho Paulo Marcos
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.990.1
Subject(s) - medicine , schistosomiasis , schistosoma mansoni , saliva , schistosoma , immunology , neglected tropical diseases , titer , antibody , gastroenterology , helminths , disease
Control constraints of schistosomiasis include the lack of diagnostic methods with high sensitivity. We develop a prospective study in southeast Brazil to standardize new sensitive diagnostic methods for Schistosoma mansoni infection. Currently, we are investigating 4 endemic areas together with 84 travelers infected in a freshwater pool. Sera, urine and saliva samples from all those patients has been used for the standardization of innovative methods, including acute, chronic, post‐treatment and myeloradiculopathy schistosomiasis patients. Comparisons are performed with eggs in feces, IgG titers, encephalomyelitis by NMR and clinical symptoms. We could diagnose acute patients 10 days post‐infection and also more than 95% of positive cases from chronic and low endemicity patients. New methods for IgG detection using purified glycoprotein or recombinant protein or peptides of 10 amino acids were superior to conventional ELISA. Best results were seen for recombinant protein with 100% of sensitivity. Finally, monoclonal antibodies were produced for improvement of the new methods by promoting direct diagnosis. Data showed 100% of sensitivity of chronic patients and 98% of acute patients. Financial support: Fapemig, CNPq, Capes, Fiocruz, Fiotec, PDTIS (Brazil). Fulbright, NIH, University of Georgia (USA). Grant Funding Source : Fapemig, CNPq, Capes, Fiocruz, Fiotec, PDTIS (Brazil). Fulbright, NIH, University of Georgia (USA).