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Metabolomic analysis of maternal energy metabolism (982.3)
Author(s) -
Rajaee Arezoo,
Murphy Aisling,
Koos Brian
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.982.3
Subject(s) - metabolite , gestation , endocrinology , medicine , excretion , pregnancy , creatinine , glycolysis , metabolomics , metabolome , urinary system , gestational age , chemistry , citric acid cycle , metabolism , biology , chromatography , genetics
Objective Maternal O2 consumption progressively increases by about 20% over the course of gestation. This study was designed to test the hypothesis that the rise in maternal O2 consumption is reflected in quantitative perturbations of metabolites in critical pathways involved in maternal energy homeostasis. Study Design Random maternal urine samples were obtained longitudinally from 16 normal gravidas with singleton pregnancies at 1) 6‐14 weeks' gestation, 24‐30 weeks' gestation, and 3) 6 weeks postpartum. The samples were analyzed on GC/MS and LC/MS/MS plateforms by Metabolon for 441 low‐molecular weight metabolites. The reported metabolites are those involved in glycolysis (glucose, lactate) and TCA cycle (alpha‐ketoglutarate, fumarate, malate, 3‐hydroxybutyrate). Concentrations were normalized to creatinine and expressed as scaled intensity. The log transformed data was analyzed by ANOVA with repeated measures to identify biochemicals that differed significantly between experimental groups. Data are expressed as fold‐changes between compared groups. Differences were significant at p<0.05. Results Compared to postpartum (PP) values, urinary excretion of glucose and lactate was increased significantly by 26 ± 1.7 (SD) weeks' gestation (late pregnancy, LP) but not at 9 ± 2.3 weeks (early pregnancy, EP), as shown in Table 1. Malate, fumarate, 3‐hydroxybutyrate, and alpha‐ketoglutarate excretion also increased substantially with gestational age. Conclusion The results are consistent with a substantial increase in maternal metabolic fluxes related to glycolysis and the TCA cycle. BHBA, 3‐hydroxybutyrate. *p蠄0.05 Table 1. Fold‐changes for urinary metabolite ratios between groups.Ratios Glucose Lactate Fumarate Malate BHBA α‐ketoglutarate PP:EP 0.71 0.59 0.98 0.67* 0.81 1.71 PP:LP 0.05* 0.13* 0.49* 0.21* 0.26* 0.23* LP:EP 14.13* 4.69* 2* 3.13* 3.16* 7.53*