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All‐ambient processing of human blood samples: complete RNA and DNA stabilization with an automated workflow (981.10)
Author(s) -
Liberal Vasco,
MullerCohn Judy,
Muller Rolf,
Whitney Scott
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.981.10
Subject(s) - rna , dna , workflow , nucleic acid , computational biology , sample (material) , microrna , nucleic acid methods , whole blood , gene , computer science , biology , chemistry , chromatography , database , genetics , immunology
Gene expression profiles from blood samples are increasingly used to diagnose and monitor disease, requiring reliable preservation of RNA during sample collection and shipment. The highly dynamic transcription profiles can change rapidly after blood collection, potentially affecting interpretation of gene expression and ultimately dictate inadequate treatment. Biomatrica has developed a collection device for whole blood, which completely stabilizes the RNA in blood cells and, coupled with a robust RNA purification kit, provides a complete solution for all‐ambient blood sample processing. RNA isolation can be performed with multiple automated platforms, allowing an easy workflow and excellent results for low to high throughput sample processing. For multiple studies, both DNA and RNA profiles from blood samples are needed, complicating logistics especially when large sample sets are required. Isolation of RNA and DNA from the same sample is ideal, since the use of separate samples could introduce operation errors as well as variation of the results. In order to accommodate the need for RNA and DNA isolations from the same blood sample stabilized in RNAgard Blood Tubes, we have developed an automated workflow for sequencial RNA and DNA purification, allowing for purification of both pathogen and host nucleic acids from the same stabilized blood sample. Biomatrica’s ambient nucleic acid preservation technology, coupled with automated purification, can serve as the front end for research and molecular diagnostics, delivering accurate gene expression patterns and prove of extreme value for improved biomedical research and better patient treatment.