Premium
Human cathelicidin hCAP‐18/LL‐37 expression and subcellular localization in LNCaP and PC3 cell line models of prostate cancer: effects on growth, migration, and invasion (978.1)
Author(s) -
Stone Sophia,
Craig Maria,
Deeble Paul
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.978.1
Subject(s) - lncap , biology , cathelicidin , cell culture , western blot , microbiology and biotechnology , cancer research , prostate cancer , innate immune system , cancer , immune system , immunology , biochemistry , genetics , gene
Human cathelicidin hCAP‐18 is an antimicrobial protein that is proteolytically processed to its active form, LL‐37, an alpha‐helical immune defense peptide. LL‐37 binds extracellular DNA and enhances cellular uptake of bacterial DNA containing unmethylated CpG motifs to activate the innate immune response. hCAP‐18 overexpression has been associated with disease progression in multiple epithelial cancers, and proliferative and pro‐metastatic effects of LL‐37 on cancer cells may contribute to a more aggressive tumor phenotype. We used RT‐PCR and western blot assays to characterize hCAP‐18 expression in LNCaP (androgen‐responsive) and PC3 (androgen‐independent) prostate cancer cell lines. We detect hCAP‐18 mRNA expression by RT‐PCR in both LNCaP and PC3 cell lines and observe higher expression in PC3 than in LNCaP cells using western blotting. Internalization and subcellular localization of LL‐37, alone and complexed with CpG oligonucleotides, were also investigated using immunofluorescence and western blot analyses. We find that LL‐37 appears to localize in endocytic vesicles and perinuclear regions of cells, and previous work by this group has shown cell line‐specific LL‐37 stimulation of growth (PC3), migration (PC3), and invasion (LNCaP). This work was supported by a Virginia Foundation of Independent Colleges Summer Undergraduate Research Fellowship.