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Bioactivity guided isolation of a novel protein from Moringa oleifera with antimicrobial activity (975.9)
Author(s) -
Ahmed Sibtain,
Jamil Amer
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.975.9
Subject(s) - moringa , antimicrobial , traditional medicine , antifungal , isolation (microbiology) , bacteria , polyacrylamide gel electrophoresis , pathogenic bacteria , chemistry , biology , microbiology and biotechnology , biochemistry , food science , medicine , enzyme , genetics
Natural products discovered from medicinal plants have provided numerous clinically used medicines. Moringa oleifera , a multipurpose plant first discovered in Asia and Africa, is now known worldwide for its nutritional and medicinal benefits as well as its industrial uses. This study describes the activity‐guided isolation and purification of a novel antimicrobial protein from Moringa oleifera . Purification was carried out by (NH 4 ) 2 SO 4 precipitation, gel filtration and ion‐exchange chromatography. Antifungal and antibacterial activities were determined after each purification step. SDS‐polyacrylamide gel electrophoresis revealed that the purified protein had a molecular mass of 15‐kDa. The protein possessed a strong and broad‐spectrum antimicrobial activity against pathogenic bacteria and fungi. It may be a future target for the development of drugs with strong antimicrobial activities. Grant Funding Source : University of Agriculture Faisalabad, Pakistan