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Effect of a combined fraction of protease inhibitor and lectins on hematopoietic system in rats (975.7)
Author(s) -
MorenoCelis Ulisses,
FerrizMartinez Roberto,
AndradePortillo Veronica,
LopezMartinez Josue,
ArandaVargas Paola,
NoriegaGiron Hector,
GuerreroCarrillo Maria De Jesus,
BlancoLabra Alejandro,
RodriguezMendez Adriana Jheny,
GarciaGasca Teresa
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.975.7
Subject(s) - spleen , protease , haematopoiesis , bone marrow , body weight , in vivo , white pulp , medicine , biology , endocrinology , immunology , pharmacology , chemistry , biochemistry , enzyme , stem cell , microbiology and biotechnology
Studies from our group have shown that lectins and protease inhibitors (PIs ) of Tepary beans have individual effects on colon cancer cells. In order to know the in vivo effects of a combined fraction of lectins and protease inhibitor (LIP‐60), alterations in the hematopoietic system were determined. Sprague Dawley rats of 3 weeks old were administered intragastrically with LIP‐60 (100 mg/kg body weight) for 28 days. Blood samples were obtained on 0, 1, 3, 9, 14, 19 and 24 days, animals were sacrificed at day 30 and spleen, thymus and femur bone marrow were obtained for histopathological analysis. Body weight and food consumption decreased were observed respect to control group. Complete blood count showed decrease in lymphocytes while granulocytes increased, suggesting allergic process. Histopathological analyzes showed reduced white pulp of the spleen, related with an increase in the release of mature leukocytes. The results suggest that LIP‐60 provoke allergy‐like response. Grant Funding Source : CONACYT FOMIX (QRO‐2011‐C02‐175340)