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Lingonberry ( Vaccinium vitis‐idaea ) anthocyanins protect cardiomyoblasts against ischemia‐reperfusion injury (973.1)
Author(s) -
Isaak Cara,
O Karmin,
Siow Yaw L
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.973.1
Subject(s) - anthocyanin , apoptosis , antioxidant , vaccinium , chemistry , phosphorylation , antioxidant capacity , berry , reperfusion injury , pharmacology , food science , ischemia , biochemistry , botany , biology , medicine
Lingonberry contains more antioxidants and anthocyanins than most berries and consumption of these phytochemicals has been shown epidemiologically to reduce risk of cardiovascular disease. Since the mechanisms of protection against cellular injury require elucidation, this study examined the effects of complete lingonberry, anthocyanin‐enriched, and phenolic‐enriched extracts on apoptosis in H9c2 rat cardiomyoblasts during simulated ischemia‐reperfusion (IR). This model induced a 20‐fold increase in nuclei condensation and JNK phosphorylation and a 2‐fold increase in caspase‐3 activation. Despite having low antioxidant capacity, the anthocyanin‐enriched extract and pure anthocyanins protected cells against apoptosis by significantly inhibiting IR‐induced nuclei condensation, caspase‐3 activation, and JNK phosphorylation to the same degree as the complete extract. These results indicate that anthocyanins may exert anti‐apoptotic effects via mechanisms unrelated to their antioxidant capacity. This research highlights the effects of dietary anthocyanins on cell signaling in IR injury and bolsters the body of evidence for the health benefits of anthocyanin‐rich foods. (AAFC Project ID 1129) Grant Funding Source : Supported by AAFC