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Investigating effects of consuming different protein and/or oil sources on progression and severity of polycystic liver disease in young female PCK rats (959.9)
Author(s) -
Nanda Nainika,
Maditz Kaitlin,
Oldaker Chris,
Livengood Ryan,
Benedito Vagner,
Tou Janet
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.959.9
Subject(s) - endocrinology , medicine , casein , polyunsaturated fatty acid , biology , inflammation , fibrosis , growth factor , cyst , soybean oil , receptor , biochemistry , pathology , fatty acid
Polycystic Liver Disease (PLD) is characterized by multiple cyst formation increasing liver size, structural damage, and loss of function. There are no effective cures for PLD, but diet may be a potential therapy. The objective of this study was to investigate whether soy protein isolate (SPI) and/or dietary omega‐3 polyunsaturated fatty acid (n‐3 PUFA) supplementation attenuates PLD progression and severity. Young (age 28 d) female PCK rats, a genetic model of PLD, were fed diets (n=12/group) consisting of casein + corn oil (Casein + CO), casein + soybean oil (Casein + SO), SPI + soybean oil (SPI + SO), or SPI + 1:1 soybean/salmon oil blend (SPI + SB) for 12‐weeks. Liver weights were not significantly different among the diet treatment groups. Histological evaluation showed no significant differences in cyst effacement and liver fibrosis between diet groups. Rats fed SPI+SB diet had the highest (P=0.03) cyst obstruction and interstitial inflammation. Gene expression by real time quantitative polymerase chain reaction (RT‐qPCR) showed no significant differences (P>0.05) in fibrosis and inflammation factors: peroxisome proliferator‐activated receptor gamma (PPARγ), mammalian target of rapamycin (mTOR), mitogen‐activated protein kinase (MAPK1), transforming growth factor beta (TGFβ), and epidermal growth factor (EGF). Furthermore, serum liver function measurements showed no significant differences among diet groups. Based on the results, dietary supplementation of SPI and n‐3 PUFA had no effect on progression or severity of PLD.