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Live‐cell imaging study on anti‐adipogenic and apoptotic effects of a polyphenolic resveratrol during adipocyte differentiation (959.19)
Author(s) -
Ihara Hayato,
Ogawa Mikako,
Magata Yasuhiro
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.959.19
Subject(s) - resveratrol , adipocyte , adipogenesis , apoptosis , adipose tissue , chemistry , microbiology and biotechnology , medicine , endocrinology , biology , biochemistry
In the present study, using fluorescent live‐cell imaging system, we demonstrate an apoptotic effects of resveratrol, a polyphenolic compound present in grapes and other foods, on adipocyte differentiation. We hypothesize that resveratrol inhibits adipocyte differentiation through an induction of apoptosis in early‐stage cells of adipocyte conversion. Adipocyte conversion and induction of apoptosis of 3T3‐L1 cells were observed using fluorescent dyes, BODIPY and Alexa568‐conjugated annexin V, respectively. Time‐lapse cell imaging was performed using BioStation system (Nikon Tokyo), which consists of CO2 incubator incorporating fluorescent microscopy with CCD camera. Resveratrol treatments during differentiating stage induced apoptosis not in mature adipocytes but in early‐stage cells of adipocyte conversion, which resulted in a dramatic inhibition of adipogenesis. Even in differentiated 3T3‐L1 adipocytes (Day 6), mature adipocytes did not undergo apoptosis in response to 24 hr‐treatments of resveratrol, whereas newly differentiating cells did. Next, we tried to elucidate anti‐adipogenic effects of resveratrol in vivo, ob/ob mice were treated with resveratrol by daily oral administration (50 mg/kg B.W.). After 1‐month treatment, 10% body weight loss was observed in these obese mice. Furthermore, CT scan before and after resveratrol administration showed that there were no clear regional differences in the reduction of adipose tissue mass, but abdominal subcutaneous adipose tissue seem to be reduced by these treatments. These results suggest that resveratrol reduce adipose tissue mass in part through an induction of apoptosis in the early‐stage cells of adipocyte differentiation.