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Prevention of vascular disease of vegetable oils in HUVEC (959.15)
Author(s) -
Kim Kyoung Kon,
Kim Dae Jung,
Kang Yun Hwan,
Lee Sung Mee,
Choe Myeon
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.959.15
Subject(s) - ginseng , pepper , vegetable oil , chemistry , nitric oxide , food science , cell growth , cell , traditional medicine , horticulture , biochemistry , biology , medicine , pathology , alternative medicine , organic chemistry
To confirmation of anti‐atherosclerosis effect by treatment of various vegetable oils, we determined the activity of cell proliferation, cell migration and NO production in HUVECs. The results of proliferation assay on vegetable oil treated HUVEC showed that, TNF‐α increased the cell proliferation and the pine nuts 0.1 μl/ml, safflower seeds 0.1 μl/ml, ginseng seeds 0.1 μl/ml inhibited the cell proliferation activity by 89.4%, 90.34%, and 91.4%, respectively, but, other vegetable oil did not affected. In cell migration assay, classified the cultured cell plates with the tip scratches and then treated with TNF‐α to stimulate cell migration. Red pepper seed, green tea seeds, citron seeds, and ginseng seeds oils of 12 vegetable oils inhibited TNF‐α‐stimulated cell migration. Nitric oxide synthase activation effect of vegetable oils was measured by NO production assay. Ginseng seeds 0.1 μl/ml, safflower seeds 0.1 μl/ml, chinese pepper 0.1 μl/ml increased the NO production by 131.43%, 129.63%, and 129.52%, respectively, but other oils did not affected. Therefore, our results suggest that further studies are required to verify the prevention of vascular disease by vegetable oil.