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Comparison of biological activities of the leaf and root extracts of Smilax china L. (959.11)
Author(s) -
Kang Yun Hwan,
Kim Dae Jung,
Kim Tae Woo,
Kim Kyoung Kon,
Choe Myeon
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.959.11
Subject(s) - chemistry , flavonoid , food science , biochemistry , antioxidant
This study was carried out to compare the biological activities of leaf and root water extracts of Smilax china L. (SC) by measuring the total polyphenol and flavonoid contents, anti‐oxidant activity, inhibitory effect on α‐glucosidase, and anti‐inflammatory gene expression. The total polyphenol and flavonoid contents of SC leaf (SCLE) and root (SCRE) water extracts were 127.93 mg GAE/g and 39.50 mg GAE/g and 41.99 mg QE/g and 1.25 mg QE/g, respectively. The anti‐oxidative activities of SCLE and SCRE were measured using 1,1‐diphenyl‐2‐picrylhydrazyl (DPPH) and 2,2′‐azino‐bis(3‐ethylbenzothiazoline‐6‐sulfonic acid) diammonium salt (ABTS) radical scavenging activity assay and reducing power assay. Both SCLE and SCRE scavenged radicals in a concentration‐dependent manner, and SCLE showed stronger radical scavenging activity and reducing power than those by SCRE; however, the activities of both SCLE and SCRE were lower than that of ascorbic acid. Compared to the anti‐diabetic drug acarbose, which was used as a positive control, in SCLE and SCRE, the α‐glucosidase inhibition activities was low, nevertheless, the activity of SCLE was 3.7 fold higher than that of SCRE. Finally, the SCLE significantly decreased the LPS‐induced cytokines, iNOS, and COX‐2 mRNA expression in RAW264.7 cells, indicating anti‐inflammatory activity. These results indicate that SCLE might be a potential candidate as an anti‐oxidant, anti‐diabetic, and anti‐inflammatory agent.