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Hexane fraction of Costus afer Ker Gawl leaf inhibited mitochondrial permeability transition, F1F0 ATPase and scavenged nitric oxide and hydrogen peroxide (957.1)
Author(s) -
Anyasor Godswill,
Onajobi Funmilayo,
Osilesi Odutola,
Adebawo Olugbenga
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.957.1
Subject(s) - chemistry , nitric oxide , mitochondrial permeability transition pore , hydrogen peroxide , spermine , in vivo , atpase , nitric oxide synthase , biochemistry , apoptosis , enzyme , biology , programmed cell death , organic chemistry , microbiology and biotechnology
Recent implication of mitochondria in the etiology of inflammatory diseases especially rheumatoid arthritis led to the evaluation of in vitro and in vivo inhibitory effects of an indigenous medicinal plant, Costus afer leaf hexane fraction (CAHFL) on mitochondrial permeability transition (MPT), F1F0 ATPase, nitric oxide (NO•) and hydrogen peroxide (H2O2) using spectrophotometric methods. Results showed that 10, 20, 40 and 60 µg/ml CAHFL significantly (P<0.05) inhibited Ca2+ induced MPT in a concentration dependent manner by 85.66%, 86.93%, 87.47% and 90.47% respectively when compared with untreated mitochondria in vitro. Spermine potentiated CAHFL MPT inhibition by 88.20%, 90.20%, 92.12% and 93.51% respectively. The In vivo study showed that 50, 100 and 250 mg/kg body weight CAHFL significantly (P<0.05) inhibited MPT in arthritic rats by 52.22%, 61.11% and 65.0% respectively in a dose dependent manner compared with control group (untreated) in 7 days. Concentrations of 10, 20, 40, 60 and 80 µg/ml CAHFL significantly (P<0.05) reduced F1F0 ATPase activity in a concentration dependent manner with 3.93 ± 0.01, 3.88 ± 0.04, 3.63 ± 0.07, 3.51 ± 0.05 and 3.49 ± 0.04 mg/ml phosphate (Pi) liberated respectively compared with 10 µg/ml 2,4‐dinitrophenol (6.60 ± 0.03 mg/ml Pi.). CAHFL scavenged NO• and H2O2 in a concentration dependent manner with an IC50¬¬ of 1.77 mg/ml and 0.33 mg/ml respectively. These data indicated that CAHFL possess the ability to protect mitochondria from damage which may account for its anti‐inflammatory action, further strengthening the claim that CAHLF could be a candidate for anti‐inflammatory drug development. Grant Funding Source : Babcock University research grant [BU/RIC/003]