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Mitochondrial membrane lipidome defines yeast longevity (956.2)
Author(s) -
Titorenko Vladimir,
Beach Adam,
Richard Vincent,
Leonov Anna,
Piano Amanda,
Feldman Rachel
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.956.2
Subject(s) - lipidome , mitochondrion , inner mitochondrial membrane , biology , yeast , microbiology and biotechnology , phospholipid , longevity , biochemistry , membrane , lipidomics , genetics
Lithocholic bile acid (LCA) is a potent anti‐aging natural compound that in yeast cultured under longevity‐extending caloric restriction (CR) conditions acts in synergy with CR to enable a significant increase in chronological lifespan. We investigated a mechanism underlying the longevity‐extending effect of LCA under CR. We found that exogenously added LCA enters yeast cells, is sorted to mitochondria, resides mainly in the inner mitochondrial membrane, and also associates with the outer mitochondrial membrane. LCA elicits an age‐related remodeling of phospholipid synthesis and movement within both mitochondrial membranes, thereby altering mitochondrial membrane lipidome and triggering major changes in mitochondrial size, number and morphology. In synergy, these changes in the membrane lipidome and morphology of mitochondria alter the age‐related chronology of mitochondrial respiration, membrane potential, ATP synthesis and reactive oxygen species homeostasis. The LCA‐driven alterations in these vital mitochondrial processes extend yeast longevity. In sum, our findings suggest a mechanism underlying the ability of LCA to delay chronological aging in yeast by accumulating in both mitochondrial membranes and altering their phospholipid compositions. We concluded that mitochondrial membrane lipidome plays an essential role in defining yeast longevity. Supported by NSERC of Canada.