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Acetyltransferase association and histone acetylation at the Myod1 locus during myogenic differentiation (946.13)
Author(s) -
Hamed Munerah,
Khilji Saadia,
Chen Jihong,
Li Qiao
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.946.13
Subject(s) - histone acetyltransferase , acetylation , enhancer , histone , chromatin , histone acetyltransferases , p300 cbp transcription factors , biology , genetics , transcription factor , microbiology and biotechnology , gene
Chromatin modifications can provide a useful readout for enhancer activities. However, it is unclear whether these modification marks are a cause or consequence of enhancer activation and transcription factors occupancy. We have examined in details the acetyltransferase associations and histone acetylations at different regulatory regions of the Myod1 locus. Our studies demonstrate that the histone acetyltransferase (HAT) p300 is stepwise enriched at distinct Myod1 regulatory regions during myogenic differentiation. This enrichment of p300 is associated with increased histone acetylation in a distinct pattern. Inhibition of p300 HAT activity delays myogenic differentiation, which is coupled with decreased histone acetylation at specific Myod1 regulatory regions. We show for the first time that p300 is directly involved in the early Myod1 enhancer regulation, and provide molecular insights into how p300 HAT activity and histone acetylation are related to enhancer activation and, consequently, gene transcription Grant Funding Source : supported by The Ministry of higher Education of Saudi Arabia (to MH) and CIHR grant (to QL)