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The effect of the estrogenic compounds E2 and BPA on the expression of histone modifying enzymes in two prostate cancer models (942.4)
Author(s) -
Burton Kevin,
Bajdas Alicia,
Shaw Lisa,
Morey Lisa
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.942.4
Subject(s) - prostate cancer , lncap , histone deacetylase , cancer , prostate , cancer research , cancer cell , gene expression , estrogen receptor , histone , medicine , chemistry , breast cancer , biochemistry , gene
Prostate cancer is the most common form of cancer for males and it has been shown that exposure to estrogenic compounds can increase the likelihood of developing prostate cancer. The goal of this study is to determine the effect of physiological doses of two estrogenic compounds, estradiol (E2) and bisphenol A (BPA), on the expression of histone modifying enzymes (HMEs) in prostate cancer cells. Alterations to the expression of HMEs could potentially lead to global changes in gene expression that may play an important role in the development of prostate cancer due to increased exposure to E2 or BPA. To determine the role of these estrogenic compounds in prostate cancer, two prostate cancer cell lines, LNCaP and PC3, were treated with E2 or BPA alone or in combination with ICI, an estrogen receptor antagonist. All treatments were carried out using physiological conditions. We then isolated RNA from the cells and analyzed changes in mRNA expression of SIRT1, a histone deacetylase, and SET8, a histone methyltransferase, using semi‐quantitative RT‐PCR. The results of our experiments show that the expression of the two HMEs being studied was altered by treatment with E2 or BPA alone as well as in combination with the ICI. We also observed different changes in gene expression of the HMEs between the two cell lines. These results indicate that the estrogenic compounds E2 and BPA do affect the expression of HMEs in two different prostate cancer models, and therefore demonstrate a complex effect in prostate cancer.