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Cissus quadrangularis has anti‐oxidant and anti‐inflammatory properties: in vitro and in vivo studies (922.15)
Author(s) -
Navarro Jorge,
Espinosa Joanna,
Das Kishore,
Faraji Bahram,
InnisWhitehouse Wendy,
Wei SunGen,
Banu Jameela
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.922.15
Subject(s) - oxidative stress , in vivo , inflammation , reactive oxygen species , apoptosis , proinflammatory cytokine , pharmacology , osteoblast , osteoporosis , diabetes mellitus , chemistry , medicine , in vitro , endocrinology , biology , biochemistry , microbiology and biotechnology
Oxidative stress in cells increases the level of reactive oxygen species (ROS), causing cellular damage and inflammation, leading to apoptosis of cells. It plays a major role in different diseases including obesity and diabetes. One of the complications that results from obesity and diabetes is osteoporosis, a disease that makes bones fragile and increases the risk of fractures. ROS inhibits osteoblast differentiation, decreasing bone formation leading to bone loss. Cissus quadrangularis (CQ) , a herb used in Ayurvedic medicine, has bone protective properties and is reported to decrease oxidative damages induced by obesity. We used mouse osteoblastic cells (MC3T3) to determine the anti‐oxidative effects of CQ. The cells were assessed for ROS production after determining the optimal concentration and time period of treatment with CQ. Increased ROS production can cause inflammation and activate the apoptotic pathway leading to cell death. We also completed an in vivo study using rats and measured the pro‐inflammatory cytokines in the serum after 4 months of feeding diet containing CQ. Results demonstrate significant inhibition of ROS and caspase 12 in CQ treated cells. We also found that caspase 12 levels are decreased in the presence of CQ. In vivo results showed decreased levels of IL‐1α and IL‐6 in the serum. These results suggest that CQ can protect bone as an anti‐oxidatant and anti‐inflammatory agent. Grant Funding Source : Undergraduate research Inititiative, NSF ADVANCE