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Jagged1 is essential and sufficient for osteoblast development during maxillary ossification (921.2)
Author(s) -
Hill Cynthia,
Yuasa Masato,
Schoenecker Jon,
Goudy Steven
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.921.2
Subject(s) - intramembranous ossification , ossification , osteoblast , runx2 , microbiology and biotechnology , anatomy , jag1 , biology , chemistry , in vitro , signal transduction , notch signaling pathway , genetics
Maxillary hypoplasia occurs due to insufficient maxillary intramembranous ossification, leading to poor dental occlusion, respiratory obstruction and cosmetic deformities. Conditional deletion of Jagged1 (Jag1) in cranial neural crest (CNC) cells using Wnt1‐ cre ( Jag1 CKO) led to maxillary hypoplasia characterized by intrinsic differences in bone morphology and density using μCT evaluation. Jag1 CKO maxillas had altered collagen deposition, delayed ossification, and reduced expression of early and late determinants of osteoblast development during maxillary ossification. In vitro bone cultures on Jag1 CKO mouse embryonic maxillary mesenchymal (MEMM) cells demonstrated decreased mineralization that was also associated with diminished induction of osteoblast determinants. BMP receptor expression was reduced in the Jag1 CKO MEMM cells suggesting that these cells were unable to respond to BMP‐induced differentiation. Jag1 ‐Fc rescued in vitro mineralization and osteoblast gene expression changes. These data suggest that Jag1 signaling in CNC‐derived MEMM cells is required for osteoblast development and differentiation during maxillary ossification. Grant Funding Source : 5K08DE17953‐5