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Human embryonic larynx morphogenesis: three‐dimensional and multiplanar study (918.26)
Author(s) -
Labrouse Marc,
Tonnelet Romain,
Renard Yohann,
Micard Emilien,
Duparc Fabrice,
Delmas Vincent,
Braun Marc
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.918.26
Subject(s) - larynx , anatomy , epiglottis , segmentation , trichrome , morphogenesis , biology , computer science , h&e stain , medicine , pathology , artificial intelligence , staining , gene , biochemistry
The purpose of this study was to describe the human larynx embryology, using three‐dimensional reconstructions from seven human embryos histological sections, and to compare our results with literature. Seven human embryos from two historic French collections (Paris‐Descartes University's Delmas‐Rouvière collection and Rouen University's Tardif collection) stained with hematoxylin & eosin or Masson's trichrome were reclassified according to the Carnegie Atlas criteria (CS (Carnegie stages) 17, 18, 19, 21 and 22). A local made computer software developed by IADI lab (INSERM U947, Nancy) allowed automatic slices selection, automatic rigid registration, and dust filtering. Once registration had been completed, DICOM files were used with ORS Visual software (v1.5, Montreal), which allowed multiple display and post‐processing possibilities (3D VR; orthogonal/curved multiplanar reformation). Due to large size files, image processing was performed on PC with Windows 7 (64 bit) and 20 GB RAM. Three‐dimensional and multiplanar study of the larynx morphogenesis was possible with appropriate thresholding. In the first stages of this study, the early laryngeal aditus was visible. Two posterior elevations, the arytenoid swellings, one anterior, the epiglottis, and the vocal folds were seen in the last stages. Human embryonic larynx morphogenesis works are very rare because of the smallness of this anatomical structure. Our study, using historic human embryos histological sections has shown the larynx growth. This demonstration was completely novel and had never been published previously, to our knowledge. Indeed, our method is fully respectful towards the microscopic structure unlike methods using Amira© segmentation and triangulation process. The precise knowledge of this morphogenesis will help to better understand congenital malformations.