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Y‐chromosome amelogenin isoform peptides found in mature dental enamel (918.1)
Author(s) -
Gerlach Raquel,
Molina Gabriela,
Issa Joao,
Sosovicka Mark,
Line Sergio,
Vieira Alexandre
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.918.1
Subject(s) - amelogenin , enamel paint , gene isoform , dental enamel , tooth enamel , biology , y chromosome , chemistry , microbiology and biotechnology , genetics , dentistry , gene , medicine
The dental enamel is the hardest and most calcified tissue in the human body, and most of its structure is unaltered during the time the teeth stay in mouth. The best known enamel specific structural proteins are amelogenin, ameloblastin and enamelin. The ability to recover proteins has enormous value in many fields where the study of proteins preserved from the past could give specific information on diet, life style, and even of biochemical evolution of the proteins themselves, since such information is not encoded in the DNA. So far, only X‐chromosome amelogenin peptides (AMELX) were recovered from mature enamel. Since amelogenin is encoded in the X and Y chromosomes in humans, peptides specific for the Y chromosome might exist in the mature enamel, but they have never been found. In this study we aimed at recovering peptides from male and female teeth, with the objectives of 1) finding Y‐encoded amelogenin peptides (if expressed at all, which is not known at the protein level) and 2) describing the aminoacid sequences of mature dental enamel peptides. Results showed that the Y‐specific amelgonenin (AMELY) sequence WYQSMIRPPY is found in male teeth and that with the enamel etch technique used here followed by nLC/MS/MS, dozens of peptides that are specific for enamel proteins were found (3 AMELX isoforms, 1 AMELY isoform, ameloblastin and enamelin). This report opens a myriad of possibilities for studying both present and long‐gone species, since dental enamel is a well preserved repository where such peptides can be detected. Grant Funding Source : Supported by FAPESP (Brazil)