Premium
The effects of insulin on gonadotropin‐releasing hormone secretion (911.1)
Author(s) -
Herrera Danny,
DiVall Sara,
Wolfe Andrew
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.911.1
Subject(s) - endocrinology , medicine , gonadotropin releasing hormone , insulin , receptor , hormone , radioimmunoassay , pulse (music) , biology , luteinizing hormone , physics , detector , optics
The purpose of this study was to determine the role of insulin signaling in gonadotropin‐releasing hormone (GnRH) pulse generation. The Cre‐lox system was used to generate mice without insulin receptors in GnRH neurons (GnRH Cre+ IR fl/fl; GnRHIRKO). Hypothalami from female GnRHIRKO or littermate controls with intact insulin receptors in GnRH neurons (GnRH Cre‐), in the lean or diet induced obesity (DIO) state were incubated in static culture. Media collected every seven minutes was subjected to radioimmunoassay for GnRH. The pulse pattern in the control DIO state was visually less regular than other states. GnRH pulse amplitude was not different between GnRHIRKO or control mice in the lean or DIO state. Pulse frequency was trended lower in the control DIO state than in lean controls or GnRHIRKO lean or DIO hypothalami. However, this result was not statistically significant. Pulse interval was also longer in the control DIO state compared to the control lean or GnRHIRKO hypothalami. Insulin signaling in the GnRH neuron may play a role in GnRH pulse generation in the DIO but not the lean state. Grant Funding Source : Supported by: NIH K08HD056139 and APS Undergraduate Summer Research Fellowship